Part of The mechanism of the dextran-induced red blood cell Aggregation. The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). 2009;8(2):1739. An undergraduate physical chemistry experiment. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. Materiaux 1-5. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea.
dextran WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)).
Fluorescent and Biotinylated DextransSection 14.5 For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. In the case of the work performed in references [34, 35, 36] they studied using the number average molecular weight, which rearranges the value of a and forces it to enter into unbranched or linear molecules, on the other hand, if molecular weights are used the value would be less than 0.5. Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. Drug Deliv. Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. Soc., 16, 3A, 426-433. Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955).
Cell permeabilization using a non-thermal plasma - IOPscience Kano S, Todo H, Furui K, Kenichi S, Tokudome Y, Hashimoto F, Kojima H, Sugibayashi K. Comparison of several reconstructed cultured human skin models by microscopic observation: their usefulness as an alternative membrane for skin in drug permeation experiments. 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861.
Radius Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. Recent Pat. Advances in chemistry series Washington, DC: American Chemical Society., pp. Naik A, Kalia YN, Guy RH. Intrinsic viscosity of bovine serum albumin conformers. The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05).
Small Intestinal Permeability and Gut-Transit Time Determined Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). Advances in Carbohydrate Analysis, 1, 63-144. Differences were considered statistically significant at p <0.05. Citation-(RIS
The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. International Journal of Pharmaceutics, 277, 99-104. If there are particles in the sample, the incident laser light gets scattered in all directions. Carbohydrate Polymers 88 373-381. Journal of Colloid Science, 31, 308-328. 2008;28(5):64557. Biophysical Journal, 27, 177-181. Studies carried out at different ionic strength you can see them in the references [64, 65] and works with different solvents in the references [66, 67]. Environ Toxicol Pharmacol.
Dextran Carbohydrate Polymers, 74, 372-378.
Dextran A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight.
The genus Weissella: taxonomy, ecology and biotechnological potential.
dextran hydrodynamic Normally, the skin loses very little water loss whereas damaged skin loses more [22]. Carbohydrate Polymers 87, 405-410. Food Sci Biotechnol. A solvent at this temperature is called a theta solvent [53]. The hydrodynamic radius of 2-NBDG glucose was too small to measure. Journal of Controlled Release, 119, 5-24. Indo AM J Pharm. FOIA Chen Rong Huei; Min Lang Tsai (1998). Polysaccharides-based nanoparticles as drug delivery systems. (2008). 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea).
Dextran 670 GPC Standard | Pharmacosmos Dextran Epub 2017 Jun 1. Intrinsic viscosity-molecular weight relationship for chitosan. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. A single frequency laser is directed to the sample contained in a cuvette. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. Ed., 9(2), 85-90. 43-1, 115-147. 4K and M.W. This site needs JavaScript to work properly. 2008;26(11):12618. Learn More. Molecular weight, molecular weight distribution and molecular size of a native dextran. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. Google Scholar. 1984;17(6):11703. In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens, Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging, Isolation of lactic acid bacteria starters from, Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. California Privacy Statement, TEWL measurements were performed before and 30min after microporation. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). By using this website, you agree to our Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Alternatives to animal testing: a review. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. Before Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Crini, G. (2005). Viscotek GmbH, Europe. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. WebDextran 40 Pharmaceutical Quality. Carbohydrate Polymers, 68, 477-488. (2013). Drug deliv. Kasaai, Mohammad R. (2007). Eng. All authors read and approved the final manuscript. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. The slope is a value and intercept is equal to the ln kMH value [47]. The human skins were from the back or thigh of a 24-year-old woman. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish
Hydrodynamic Radius The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). Microporation applications for enhancing drug delivery. Biomaterials Research Leiva, Angel; Muoz, Natalia; Urza, Marcela; Gargallo, Ligia; Radic, Deodato (2010). These results could be attributed to the differences in the in vivo and in vitro skin nutrients supply; with in vivo studies, the skin tissue is supplied nutrients directly from the blood vessels, but the human cadaver skin is supplied from the media, therefore, the skin tissue regeneration efficiency is low [26,27,28].
What is dextran? Application areas - TdB Labs AB Hydrodynamic Terms and Conditions, Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). Pharm Res. 2012;101(8):28619. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. Anal. Expert Rev Med Devices. Pharm Res. High Manufacturing Standards of Molecular Probes Dextrans. The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. He, L.; Niemeyer, B. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. The solution was measured using an Anton Paar DMA35N densitometer. (1937). Microporation techniques for enhanced delivery of therapeutic agents. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Arond L.H. Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). Keywords: FITC dextran; intestinal permeability; transit time. There is a deviation from linearity after 580kDa [69, 70]. MeSH European Biophysical Journal, 36, 85-94. Skin barrier disruption by acetone: observations in a hairless mouse skin model.
hydrodynamic 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth PubMedGoogle Scholar. In addition, we evaluated the regeneration of SC after microporation. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. dextran hydrodynamic radius. The receptor phase was collected immediately (time 0) and 2, 4, 8, 12, and 24h. This receptor phase solution was transferred to a 96-well plate and the skin-permeated FITC-dextran was measured using a microplate reader (Molecular Devices, Co. Ltd., Sunnyvale, CA, USA) at 405nm. RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. J Cosmet Laser Ther. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. Carbohydrate Polymers, 38, 267-271. of different origins. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). (2007). When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. Cookies policy. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Amersham Biosciences, AA Edition, www.amershambiosciences.com.
Hydrodynamic Smith, David R.; Raymonda, John W. (1972). Dextran. For Research Use Only. Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. This result could reflect the presence of some aggregated species within the lysozyme sample solution. There is a formula that used to calculate the radius (in Angstrom) from the molar mass (in g/mol) of dextran, a=0.33*(MM^0.46) where, a and MM are Ind. ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. They concluded that a power law behavior seems to be approached only in the small molecular weight region. Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. Human skins (Full-thickness human skin) were supplied by HansBiomed. Unable to load your collection due to an error, Unable to load your delegates due to an error. Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. Nair SS. In fact, short chains do contain minor branching points. 2003;10:33847. Federal government websites often end in .gov or .mil. Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. One day before the experiment, the skin was stabilized in the skin culture medium. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. a-d Haematoxylin/eosin-stained histological sections of human cadaver skin sample and e quantified data using ImageJ for different time periods after microporation. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. 2016;9706:97061E6. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. Harding, S. E., & Simpkin, N. J. Huggins, Maurice L. (1942). Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. NCI CPTC Antibody Characterization Program. Huggins equation [41] gives the relation to the relative viscosity increase (i). Progress in Biophysical Molecules Biological, 68, 207-262.